Acridine Orange / Propidium Iodide (AO/PI) cell viability kit
AO/PI Cell Viability Kit (500 tests) – Two (2) vials of 0.5ml pre-mixed Acridine Orange/Propidium Iodide solution, the best solution for Luna-FL™ Automated Fluorescence Cell Counter. AO/PI are used together to differentiate between viable, apoptotic and necrotic cells. Where live cells display green fluorescence, apoptotic/necrotic cells stain red fluorescence. AO/PI Cell Viability Kit provides pre-mixed AO/PI solution that can be mixed with cell samples for counting on Luna-FL cell counter.
Acridine orange is a nucleic acid selective fluorescent cationic dye. It is cell-permeable,when bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). Propidium iodide (or PI) is an intercalating fluorescent dye that can be used to stain cells. When PI is bound to nucleic acids, the fluorescence excitation maximum is 535 nm and the emission maximum is 617 nm.
|Size||2 vials of 500 µl|