Anti-β-catenin (E53K) Mouse Monoclonal Antibody, 100 µl (26167) NEBA10184

Anti-β-catenin (E53K) Mouse Monoclonal Antibody, 100 µl (26167) Signal Transduction NewEast Biosciences NEBA10184
Supplier: NewEast Biosciences
Product Code: NEBA10184
Availability: In Stock
$450.00 $427.50
Qty:  
Add to Wish List Add to Compare

Background

Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.

Tested Applications

ELISA, WB, IF, IHC

Source Species

Mouse

Species Reactivity

Recognizes β-catenin(E53K), but not wild type β-catenin protein from vertebrates.

Datasheet  PDF

Western Blot

Western blot analysis of recombinant β-catenin (E53K) and wildtype proteins. Purified His-tagged β-catenin (E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-β-catenin (E53K) mouse monoclonal antibody (Cat. #NEBA10184).

Immunofluorescence

Immunofluorescence of cells expressing β-catenin proteins with anti-β-catenin (E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β-catenin (WT) plasmid (left column) or pCDNA3-GFP-β-catenin (E53K) plasmid (right column), then fixed and stained with anti-β-catenin (E53K) monoclonal antibody (Cat. #NEBA10184).

General
Gene Symbol CTNNB1, CTNNB
Applications ELISA, WB, IF, IHC
Concentration 1 mg/ml
Purity Purified from ascites
Size 100 µl
Source Species Mouse
Antibody
Immunogen A synthetic peptide from the internal region of β-catenin which includes the mutation of E53K, human origin
Clonality Monoclonal
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recommended Dilutions ELISA, 1:1000-1:5000; WB, 1:500-1:2000; IHC, 1:50-1:100
Species Reactivity Recognizes β-catenin(E53K), but not wild type β-catenin protein from vertebrates.

Write a review

Your Name:


Your Review: Note: HTML is not translated!

Rating: Bad           Good

Enter the code in the box below:



©BioAstrum Corporation. All Rights Reserved 2014.
Google+