Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
ELISA, WB, IF, IHC
Recognizes β-catenin(E53K), but not wild type β-catenin protein from vertebrates.
Western blot analysis of recombinant β-catenin (E53K) and wildtype proteins. Purified His-tagged β-catenin (E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-β-catenin (E53K) mouse monoclonal antibody (Cat. #NEBA10184).
Immunofluorescence of cells expressing β-catenin proteins with anti-β-catenin (E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β-catenin (WT) plasmid (left column) or pCDNA3-GFP-β-catenin (E53K) plasmid (right column), then fixed and stained with anti-β-catenin (E53K) monoclonal antibody (Cat. #NEBA10184).
|Gene Symbol||CTNNB1, CTNNB|
|Applications||ELISA, WB, IF, IHC|
|Purity||Purified from ascites|
|Immunogen||A synthetic peptide from the internal region of β-catenin which includes the mutation of E53K, human origin|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||ELISA, 1:1000-1:5000; WB, 1:500-1:2000; IHC, 1:50-1:100|
|Species Reactivity||Recognizes β-catenin(E53K), but not wild type β-catenin protein from vertebrates.|