V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia.
ELISA, WB, IHC
Recognizes Y253H mutated, but not wildtype of vertebrates.
Western blot analysis of recombinant Abl (Y253H) and wildtype proteins. Purified His-tagged Abl (Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-Abl (Y253H) mouse monoclonal antibody (Cat. #NEBA10177).
Immunofluorescence of cells expressing Abl proteins with anti-Abl (Y253H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl (Y253H) plasmid (right column), then fixed and stained with anti-Abl (Y253H) monoclonal antibody (Cat. #NEBA10177).
|Gene Symbol||ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl|
|Applications||ELISA, WB, IHC|
|Purity||Purified from ascites|
|Immunogen||A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||ELISA, 1:1000-1:5000; WB, 1:100-1:1000; IHC, 1:50-1:100|
|Species Reactivity||Recognizes Y253H mutated, but not wildtype of vertebrates.|