Anti-Abl (Y253H) Mouse Monoclonal Antibody, 100 µl (26154) NEBA10177

Anti-Abl (Y253H) Mouse Monoclonal Antibody, 100 µl (26154) Cancer Research NewEast Biosciences NEBA10177
Supplier: NewEast Biosciences
Product Code: NEBA10177
Availability: In Stock
$450.00 $427.50
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Background

V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia.

Tested Applications

ELISA, WB, IHC

Source Species

Mouse

Species Reactivity

Recognizes Y253H mutated, but not wildtype of vertebrates.

Datasheet  PDF

Western Blot

Western blot analysis of recombinant Abl (Y253H) and wildtype proteins. Purified His-tagged Abl (Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-Abl (Y253H) mouse monoclonal antibody (Cat. #NEBA10177).

Immunofluorescence

Immunofluorescence of cells expressing Abl proteins with anti-Abl (Y253H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl (Y253H) plasmid (right column), then fixed and stained with anti-Abl (Y253H) monoclonal antibody (Cat. #NEBA10177).

General
Gene Symbol ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl
Applications ELISA, WB, IHC
Concentration 1 mg/ml
Purity Purified from ascites
Size 100 µl
Source Species Mouse
Antibody
Immunogen A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin
Clonality Monoclonal
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recommended Dilutions ELISA, 1:1000-1:5000; WB, 1:100-1:1000; IHC, 1:50-1:100
Species Reactivity Recognizes Y253H mutated, but not wildtype of vertebrates.

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