ARF6 ( ADP- ribosylation factor 6 ) is a member of the ARF superfamily. ARF genes encode small GTPases that increase the ADP- ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulate s membrane trafficking and functions as a regulatory molecule of phagocytosis.
Anti-active Arf6 antibody recognizes active Arf6 from vertebrates.
Arf6 activation assay. Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left ) or GTPγS (lane right ). Immunoprecipitation was done with the anti-active Arf6 monoclonal antibody (Cat. # NEBA10223). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. #NEBA10020).
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Arf6|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:50-1:100|
|Species Reactivity||Anti-active Arf6 antibody recognizes active Arf6 from vertebrates.|