Anti-Active Arl1 Mouse Monoclonal Antibody, 30 µl (26924) NEBA10229

Anti-Active Arl1 Mouse Monoclonal Antibody, 30 µl (26924) Signal Transduction NewEast Biosciences NEBA10229
Supplier: NewEast Biosciences
Product Code: NEBA10229
Availability: In Stock
$450.00 $427.50
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Background

Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.

Tested Applications

IP, IHC, IF

Source Species

Mouse

Species Reactivity

Arl1 antibody recognizes active Arl1-GTP from vertebrates.

Datasheet  PDF

Immunoprecipitation/Western Blot

Arl1 Activation Assay. Purified GST-tagged Arl1 proteins were immunoprecipitated with the anti-active Arl1 monoclonal antibody (Cat. #NEBA10229) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Arl1 monoclonal antibody (Cat. #NEBA10135). Arl1 protein input control is shown in the bottom panel.

General
Gene Symbol Arl1
Applications IP, IHC, IF
Concentration 1 mg/ml
Purity Purified from ascites
Size 30 µl
Source Species Mouse
Antibody
Immunogen Recombinant Arl1 protein(active form)
Clonality Monoclonal
Isotype IgG
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recommended Dilutions IP: 1 µg for 1~2 mg total cellular proteins; IHC/IF: 1:50-1:100
Species Reactivity Arl1 antibody recognizes active Arl1-GTP from vertebrates.

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