Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.
IP, IHC, IF
Arl1 antibody recognizes active Arl1-GTP from vertebrates.
Arl1 Activation Assay. Purified GST-tagged Arl1 proteins were immunoprecipitated with the anti-active Arl1 monoclonal antibody (Cat. #NEBA10229) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Arl1 monoclonal antibody (Cat. #NEBA10135). Arl1 protein input control is shown in the bottom panel.
|Applications||IP, IHC, IF|
|Purity||Purified from ascites|
|Immunogen||Recombinant Arl1 protein(active form)|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC/IF: 1:50-1:100|
|Species Reactivity||Arl1 antibody recognizes active Arl1-GTP from vertebrates.|