Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery.
IP, IHC, IF
Arl3 antibody recognizes active Arl3-GTP from vertebrates.
Arl3 Activation Assay. Purified His-tagged Arl3 proteins (Cat. #NEBCC0063) were immunoprecipitated with the anti-active Arl3 monoclonal antibody (Cat. #NEBA10230) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Arl3 monoclonal antibody. Input controls are shown in the bottom panel.
|Applications||IP, IHC, IF|
|Purity||Purified from ascites|
|Immunogen||Recombinant Arl3 protein(active form)|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC/IF: 1:50-1:100|
|Species Reactivity||Arl3 antibody recognizes active Arl3-GTP from vertebrates.|