Anti-Active Cdc42 Mouse Monoclonal Antibody, 30 µl (26905) NEBA10212

Anti-Active Cdc42 Mouse Monoclonal Antibody, 30 µl (26905) Signal Transduction NewEast Biosciences NEBA10212
Supplier: NewEast Biosciences
Product Code: NEBA10212
Availability: In Stock
$450.00 $427.50
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Background

Small GTPases are a superfamily of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Tested Applications

IP, IHC

Source Species

Mouse

Species Reactivity

Anti-active Cdc42 antibody recognizes active Cdc42 from vertebrates.

Datasheet  PDF

Immunoprecipitation/Western Blot

IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-active Cdc42 mouse monoclonal antibody (Cat. #NEBA10212). After SDS/PAGE, the membrane filter was probed with anti-Cdc42 mouse monoclonal antibody (Cat. #NEBA10104).

Immunofluorescence

Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeablized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1:25 dilution in Invitrogen's CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1:200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld's hard set mounting medium (H-1500), and examined using a Zeiss inverted epifluorescent miscroscope.

General
Gene Symbol CDC42
Applications IP, IHC
Concentration 1 mg/ml
Purity Purified from ascites
Size 30 µl
Source Species Mouse
Antibody
Immunogen Recombinant full length protein of active Cdc42
Clonality Monoclonal
Isotype IgG
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recommended Dilutions IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:100 dilution
Species Reactivity Anti-active Cdc42 antibody recognizes active Cdc42 from vertebrates.

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