Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. #NEBA10209), these proteins were separated by SDS/PAGE and western blotted with anti-Gα13 mouse monoclonal antibody (Cat. #NEBA10100).
Staining of MEF cells (treated with PDGF) with anti-Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.
|Purity||Purified from ascites|
|Immunogen||Recombinant full length active Ga13 protein|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:100 dilution|
|Species Reactivity||Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.|