Anti-Active Gα13-GTP Mouse Monoclonal Antibody, 30 µl (26902) NEBA10209

Anti-Active Gα13-GTP Mouse Monoclonal Antibody, 30 µl (26902) Signal Transduction NewEast Biosciences NEBA10209
Supplier: NewEast Biosciences
Product Code: NEBA10209
Availability: In Stock
$450.00 $427.50
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Background

Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.

Tested Applications

IP, IHC

Source Species

Mouse

Species Reactivity

Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.

Datasheet  PDF

Immunoprecipitation/Western Blot

IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. #NEBA10209), these proteins were separated by SDS/PAGE and western blotted with anti-Gα13 mouse monoclonal antibody (Cat. #NEBA10100).

Immunohistochemistry

Staining of MEF cells (treated with PDGF) with anti-Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.

General
Gene Symbol Gna13
Applications IP, IHC
Concentration 1 mg/ml
Purity Purified from ascites
Size 30 µl
Source Species Mouse
Antibody
Immunogen Recombinant full length active Ga13 protein
Clonality Monoclonal
Isotype IgG
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recommended Dilutions IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:100 dilution
Species Reactivity Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.

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