Anti-Active Gαo Mouse Monoclonal Antibody, 30 µl (26907) NEBA10214

Anti-Active Gαo Mouse Monoclonal Antibody, 30 µl (26907) Signal Transduction NewEast Biosciences NEBA10214
Supplier: NewEast Biosciences
Product Code: NEBA10214
Availability: In Stock
$450.00 $427.50
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Background

Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.

Tested Applications

IP, IHC

Source Species

Mouse

Species Reactivity

Anti-active Gαo antibody recognizes active Gαo of vertebrates.

Datasheet  PDF

Immunoprecipitation/Western Blot

o activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. #NEBA10214). Immunoblot was with an anti-Gαo polyclonal antibody (Cat. #NEBA10012).

General
Gene Symbol Gnao
Applications IP, IHC
Concentration 1 mg/ml
Purity Purified from ascites
Size 30 µl
Source Species Mouse
Antibody
Immunogen Recombinant full length protein of active Gao
Clonality Monoclonal
Isotype IgG
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recommended Dilutions IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:100 dilution
Species Reactivity Anti-active Gαo antibody recognizes active Gαo of vertebrates.

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