Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
Anti-active Gαo antibody recognizes active Gαo of vertebrates.
Gαo activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. #NEBA10214). Immunoblot was with an anti-Gαo polyclonal antibody (Cat. #NEBA10012).
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Gao|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:100 dilution|
|Species Reactivity||Anti-active Gαo antibody recognizes active Gαo of vertebrates.|