Heterotrimeric G proteins are critical cellular signal transducers. Gαs represents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases.
Anti-active Gαs antibody recognizes active Gαs of vertebrates.
Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαs monoclonal antibody (Cat. #NEBA10213). Immunoblot was with an anti-Gαs monoclonal antibody (Cat. #NEBA10102).
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Gαs|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:100 dilution|
|Species Reactivity||Anti-active GαS antibody recognizes active Gαs of vertebrates.|