Small GTPases of the Rab- family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.
Anti-active Rab11 antibody recognizes active Rab11 from vertebrates.
Rab11 activation assay. Purified full-length Rab11 proteins were i mmunoprecipitated after treated with GDP (lane left) or GTPγ S (lane right). Immunoprecipitation was done with the anti-active Rab11 monoclonal antibody (Cat. # NEBA10224). Immunoblot was with an anti-Rab5 poly clonal antibody (Cat. #NEBA10069).
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Rab11|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:50-1:100|
|Species Reactivity||Anti-active Rab11 antibody recognizes active Rab11 from vertebrates.|