Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles.
IP, IHC, IF
Anti-active Rab3 antibody recognizes active Rab3 proteins of vertebrates.
Rab3 activation assay. Purified full-length Rab3A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab3 monoclonal antibody (Cat. #NEBA10225). Immunoblot was with an anti-Rab3A polyclonal antibody (Cat. #NEBA10028).
|Applications||IP, IHC, IF|
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Rab3A|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for ~2 mg total cellular proteins; IHC/IF: 1:100 dilution|
|Species Reactivity||Anti-active Rab3 antibody recognizes active Rab3 proteins of vertebrates.|