Anti-Active Rab35 Mouse Monoclonal Antibody, 30 µl (26922) NEBA10227

Anti-Active Rab35 Mouse Monoclonal Antibody, 30 µl (26922) Signal Transduction NewEast Biosciences NEBA10227
Supplier: NewEast Biosciences
Product Code: NEBA10227
Availability: In Stock
$450.00 $427.50
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Background

The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.

Tested Applications

IP, IHC, IF

Source Species

Mouse

Species Reactivity

Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.

Datasheet  PDF

Immunoprecipitation/Western Blot

Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab35 monoclonal antibody (Cat. #NEBA10227). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. #NEBA10047).

General
Gene Symbol RAB35
Applications IP, IHC, IF
Concentration 1 mg/ml
Purity Purified from ascites
Size 30 µl
Source Species Mouse
Antibody
Immunogen Recombinant full length protein of active Rab35
Clonality Monoclonal
Isotype IgG
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Recommended Dilutions IP: 1 µg for ~2 mg total cellular proteins; IHC/IF: 1:100 dilution
Species Reactivity Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.

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