The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.
IP, IHC, IF
Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.
Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab35 monoclonal antibody (Cat. #NEBA10227). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. #NEBA10047).
|Applications||IP, IHC, IF|
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Rab35|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for ~2 mg total cellular proteins; IHC/IF: 1:100 dilution|
|Species Reactivity||Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.|