Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic.
Anti-active Rab4 antibody recognizes active Rab4 from vertebrates.
Rab4A activation assay. Purified GST-tagged Rab4A proteins were immunoprecipitated with the anti-active Rab4 monoclonal antibody (Cat. #NEBA10226) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Rab4A polyclonal antibody. Input control is shown in the bottom panel.
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Rab4A|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for ~2 mg total cellular proteins; IHC: 1:100 dilution|
|Species Reactivity||Anti-active Rab4 antibody recognizes active Rab4 from vertebrates.|