Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates.
Rab5 activation assay. Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab5 monoclonal antibody (Cat. #NEBA10218). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. #NEBA10067).
|Purity||Purified from ascites|
|Immunogen||Recombinant full length protein of active Gao|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||IP: 1 µg for 1~2 mg total cellular proteins; IHC: 1:100 dilution|
|Species Reactivity||Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates.|