Anti-c-myc Epitope Tag Mouse Monoclonal Antibody (DBOA10004), 100 ug DBOA10004

Anti-c-myc Epitope Tag Mouse Monoclonal Antibody (DBOA10004), 100 ug Signal Transduction DBio DBOA10004
Supplier: DBio
Product Code: DBOA10004
Availability: In Stock

Anti-c-myc Epitope Tag (EQKLISEEDL) Mouse Monoclonal Antibody (DBOA10004), 100 ug

Background:

A myc tag EQKLISEEDL is a peptide tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway. 

Product datasheet:

Overview

 

Product Name  

Anti-c‐myc Epitope Tag Mouse Monoclonal Antibody (DBOA10004), 100 ug

Description

Mouse Monoclonal Antibody (DBOA10004), against c‐myc Epitope Tag, can be used in identify myc tagged fusion protein in Western blotting, immunoprecipitation immunofluorescence microscopy or other immunoassays.

Tested Applications

WB, IHC

Species Reactivities

Reacts with: c-myc tag

Immunogen     

c-myc tag peptide EQKLISEEDL

Positive Control

N/A

 

Properties

 

Form 

Freeze dried solid

Storage Instructions

Store at +4°C long term.  After reconstitution, Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage Buffer              

When reconstituted with 100 deionized water, PBS with no preservatives.

Constituent

PBS

Purity  

Purified by protein A

Clonality

Monoclonal

Clone Name

M1Y

Isotype

IgG1

Applications

The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user.

WB

1/500 - 1/2000.

IHC

1/200 - 1/1000

General
Gene Symbol MYC ; MRTL; MYCC; bHLHe39; c-Myc
Applications WB, IP
Concentration 1 mg/ml when reconstitute in 100 ul
Purity Purified from ascites
Size 100 µg
Source Species Mouse
Antibody
Immunogen N-EQKLISEEDL-C, N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C
Clonality Monoclonal
Isotype IgG1
Form Freeze dry solid
Storage Instructions Store at 4°C.
Storage Buffer Preservative: None; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, when reconstitute with 100 ul di water

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