Anti-FGF2 Mouse Monoclonal Antibody [F2G] (DBOA10001), 100 ug DBOA10001

Anti-FGF2 Mouse Monoclonal Antibody [F2G] (DBOA10001), 100 ug Signal Transduction DBio DBOA10001
Supplier: DBio
Product Code: DBOA10001
Availability: In Stock

This is a mouse monoclonal IgM antibody against humna Fibroblast Growth Factor-2.

Background

FGF2, also known as Basic fibroblast growth factor, bFGF, or FGF-β is a member of the fibroblast growth factor family [1].

In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide. It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis.

In addition, it is synthesized and secreted by human adipocytes and the concentration of bFGF correlates with the BMI in blood samples. In this study, bFGF was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase.[3]

Reference

  1.  Kim HS (1998). "Assignment of the human basic fibroblast growth factor gene FGF2 to chromosome 4 band q26 by radiation hybrid mapping". Cytogenet. Cell Genet. 83 (1-2): 73. doi:10.1159/000015129. PMID 9925931.
  2. Kühn MC, Willenberg HS, Schott M, Papewalis C, Stumpf U, Flohé S, Scherbaum WA, Schinner S. (2012). "Adipocyte-secreted factors increase osteoblast proliferation and the OPG/RANKL ratio to influence osteoclast formation.". Mol Cell Endocrinol. 349 (2): 180–188. doi:10.1016/j.mce.2011.10.018. PMID 22040599.

Product datasheet:

Overview

 

Product Name  

Anti-FGF2 Mouse Monoclonal Antibody (DBOA10001), 100 ug

Description

Mouse Monoclonal Antibody against human Fibroblast Growth Factor 2 (FGF2), (Clone F2G), 100 ug

Tested Applications

WB, IHC

Species Reactivities

Reacts with: Human FGF2

Immunogen     

Purified recombinant fragment of FGF2 expressed in E. Coli

Positive Control

N/A

 

Properties

 

Form 

Freeze dried solid

Storage Instructions

Store at +4°C long term.  After reconstitution, Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage Buffer              

When reconstituted with 100 deionized water, PBS with no preservatives.

Constituent

PBS

Purity  

Purified by precipitation

Clonality

Monoclonal

Clone Number

F2G

Isotype

IgM

Applications

The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user.

WB

1/500 - 1/2000. Predicted molecular weight: 31 kDa

IHC

1/200 - 1/1000

General
Gene Symbol FGF2, FGF-b
Applications WB, IF, IHC
Concentration 1 mg/ml when reconstituted in 100 ul
Purity Purified from ascites
Size 100 µg
Source Species Mouse
Antibody
Immunogen Human FGF2 expressed in E Coli
Clonality Monoclonal
Isotype IgM
Form Freeze dry solid
Storage Instructions Store at 4°C.
Storage Buffer Preservative: None; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, when reconstitute with 100 ul di water
Species Reactivity Human FGF2

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