Anti-GST Tag (glutathione-S-transferase) Mouse Monoclonal Antibody (DBOA10002), 100 ug DBOA10002

Anti-GST Tag (glutathione-S-transferase) Mouse Monoclonal Antibody (DBOA10002), 100 ug Cell Biology DBio DBOA10002
Supplier: DBio
Product Code: DBOA10002
Availability: In Stock

Mouse Monoclonal Antibody against Tag (glutathione-S-transferase) (DBOA10002), 100 ug

Background

A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It is fused to the N-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

Datasheet

Overview

 

Product Name  

Anti-GST (glutathione-S-transferase) Mouse Monoclonal Antibody (DBOA10002), 100 ug

Description

Anti-GST (glutathione-S-transferase) Mouse Monoclonal Antibody (DBOA10002), 100 ug, can be used to purify or identify GST fusion proteins.

Tested Applications

WB, IHC

Species Reactivities

Reacts with: GST tag

Immunogen     

GST tag peptide

Positive Control

N/A

 

Properties

 

Form 

Freeze dried solid

Storage Instructions

Store at +4°C long term.  After reconstitution, Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage Buffer              

When reconstituted with 100 deionized water, PBS with no preservatives.

Constituent

PBS

Purity  

Purified by protein A

Clonality

Monoclonal

Clone Name

G1S

Isotype

IgG1

Applications

The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user.

WB

1/500 - 1/2000.

IHC

1/200 - 1/1000

General
Applications WB, IP
Concentration 1 mg/ml when reconstitute in 100 ul
Purity Purified from ascites
Size 100 µg
Source Species Mouse
Antibody
Immunogen glutathione-S-transferase
Clonality Monoclonal
Isotype IgG1
Form Freeze dry solid
Storage Instructions Store at 4°C.
Storage Buffer Preservative: None; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, when reconstitute with 100 ul di water

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