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Anti-GST Tag (glutathione-S-transferase) Mouse Monoclonal Antibody (DBOA10002), 100 ug DBOA10002
Mouse Monoclonal Antibody against Tag (glutathione-S-transferase) (DBOA10002), 100 ug
Background
A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It is fused to the N-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Datasheet
Overview |
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Product Name |
Anti-GST (glutathione-S-transferase) Mouse Monoclonal Antibody (DBOA10002), 100 ug |
| Description |
Anti-GST (glutathione-S-transferase) Mouse Monoclonal Antibody (DBOA10002), 100 ug, can be used to purify or identify GST fusion proteins. |
|
Tested Applications |
WB, IHC |
|
Species Reactivities |
Reacts with: GST tag |
|
Immunogen |
GST tag peptide |
|
Positive Control |
N/A |
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Properties |
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Form |
Freeze dried solid |
| Storage Instructions |
Store at +4°C long term. After reconstitution, Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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Storage Buffer |
When reconstituted with 100 deionized water, PBS with no preservatives. |
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Constituent |
PBS |
|
Purity |
Purified by protein A |
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Clonality |
Monoclonal |
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Clone Name |
G1S |
|
Isotype |
IgG1 |
Applications |
The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user. |
|
WB |
1/500 - 1/2000. |
|
IHC |
1/200 - 1/1000 |
| General | |
| Applications | WB, IP |
| Concentration | 1 mg/ml when reconstitute in 100 ul |
| Purity | Purified from ascites |
| Size | 100 µg |
| Source Species | Mouse |
| Antibody | |
| Immunogen | glutathione-S-transferase |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Form | Freeze dry solid |
| Storage Instructions | Store at 4°C. |
| Storage Buffer | Preservative: None; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, when reconstitute with 100 ul di water |
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