Anti-HA Epitope Tag Mouse Monoclonal Antibody (DBOA10003), 100 ug DBOA10003

Anti-HA Epitope Tag Mouse Monoclonal Antibody (DBOA10003), 100 ug Applications DBio DBOA10003
Supplier: DBio
Product Code: DBOA10003
Availability: In Stock

Mouse Monoclonal Antibody (DBOA10003) against HA Epitope Tag , 100 ug

Background

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag YPYDVPDYA is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Reference

M Prakriya, S Feske, Y Gwack, S Srikanth, A Rao (September 2006). "Orai1 is an essential pore subunit of the CRAC channel". Harvard Medical School and the CBR Institute for Biomedical Research. doi:10.1038/nature05122.

Product datasheet:

Overview

 

Product Name  

Anti-HA Epitope Tag Mouse Monoclonal Antibody (DBOA10003), 100 ug

Description

Anti-HA Epitope Tag Mouse Monoclonal Antibody (DBOA10003), 100 ug, can be used in identify HA tagged fusion protein in Western blotting, immunoprecipitation immunofluorescence microscopy or other immunoassays.

Tested Applications

WB, IHC

Species Reactivities

Reacts with: YPYDVPDYA epitope

Immunogen     

YPYDVPDYA

Positive Control

N/A

 

Properties

 

Form 

Freeze dried solid

Storage Instructions

Store at +4°C long term.  After reconstitution, Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage Buffer              

When reconstituted with 100 deionized water, PBS with no preservatives.

Constituent

PBS

Purity  

Purified by protein A

Clonality

Monoclonal

Clone Name

H2A

Isotype

IgG2b

Applications

The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user.

WB

1/500 - 1/2000.

IHC

1/200 - 1/1000

General
Applications WB, IP
Concentration 1 mg/ml when reconstitute in 100 ul
Purity Purified from ascites
Size 100 µg
Source Species Mouse
Antibody
Immunogen YPYDVPDYA
Clonality Monoclonal
Isotype IgG2b
Form Freeze dry solid
Storage Instructions Store at 4°C.
Storage Buffer Preservative: None; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, when reconstitute with 100 ul di water

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