Anti-Human Estrogen Receptor alpha (ER-α) Mouse Monoclonal Antibody (DBOA10005), 100 ug DBOA10005

Anti-Human Estrogen Receptor alpha (ER-α) Mouse Monoclonal Antibody (DBOA10005), 100 ug Signal Transduction DBio DBOA10005
Supplier: DBio
Product Code: DBOA10005
Availability: In Stock

Anti-Human Estrogen Receptor alpha Mouse Monoclonal Antibody (DBOA10005), is a IgG monoclonal that recognizes both human and mouse ERa.  it has excellent signal to background noise ratio in IF, IHC and WB assays, out performing many popular ER antibodies on the market.

Anti-ER-α image,

Western blotting and immunofluorescence microscopy

anti-ER alpha western blotting and immunofluorescence microscopy image

Background

Estrogen receptor alpha (ER-α), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor that is activated by the sex hormone estrogen. In humans, ER-α is encoded by the gene ESR1 (EStrogen Receptor 1).[1][2][3]

Product datasheet:

Overview

 

Product Name  

Anti-Human Estrogen Receptor alpha Mouse Monoclonal Antibody (DBOA10005), 100 ug

Description

Anti-Human Estrogen Receptor alpha Mouse Monoclonal Antibody (DBOA10005), 100 ug, can be used in study ER receptor alpha expression in cultured cells or tissue samples.

Tested Applications

WB, IHC

Species Reactivities

Reacts with: human and mouse ER alpha

Immunogen     

Human ER alpha

Positive Control

N/A

 

Properties

 

Form 

Freeze dried solid

Storage Instructions

Store at +4°C long term.  After reconstitution, Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage Buffer              

When reconstituted with 100 deionized water, PBS with no preservatives.

Constituent

PBS

Purity  

Purified by protein A

Clonality

Monoclonal

Clone Name

E2R

Isotype

IgG

Applications

The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user.

WB

1/500 - 1/2000.

IHC

1/200 - 1/1000

 

General
Gene Symbol ESR1 ; ER; ESR; ESRA; ESTRR; Era; NR3A1
Applications WB, IF, IHC
Concentration 1 mg/ml when reconstitute in 100 ul
Purity Purified from ascites
Size 100 µg
Source Species Mouse
Antibody
Immunogen Human Estrogen Receptor alpha
Clonality Monoclonal
Isotype IgG
Form Freeze dry solid
Storage Instructions Store at 4°C.
Storage Buffer Preservative: None; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, when reconstitute with 100 ul di water
Species Reactivity Human and mouse ER-α

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