Anti-Orai1 Rabbit Polyclonal Antibody (Orai1-L1), 100 µl (21001) NEBA10001

Anti-Orai1 Rabbit Polyclonal Antibody  (Orai1-L1), 100 µl (21001) Signal Transduction NewEast Biosciences NEBA10001
Supplier: NewEast Biosciences
Product Code: NEBA10001
Availability: In Stock
$179.00 $170.05
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Background

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combined immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Tested Applications

ELISA, WB, IHC

Source Species

Rabbit

Species Reactivity

Orai1-L1 antibody recognizes Orai1 from human and mouse. It may also recognize Orai2 and Orai3.

Datasheet  PDF

Western Blot

Western blot analysis of Orai1 protein. Human Orai1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot in the absence (lane 1) or presence (lane 2) of antigen peptide.

Immunohistochemistry

Immunohistochemistry of endogenous Orai1 in NIH3T3 cells showing the plasma membrane staining.

General
Gene Symbol Orai1
Applications ELISA, WB, IHC
Concentration 0.5 mg/ml
Purity Immunogen affinity purified
Size 100 µl
Source Species Rabbit
Antibody
Immunogen A peptide from the extracellular loop 1 region of human Orai1
Clonality Polyclonal
Isotype IgG
Form Liquid
Storage Instructions Store at -20°C. Avoid repeated freeze / thaw cycles.
Storage Buffer Preservative: 0.01% sodium azide; Constituents: 50mM NaHCO3-Glycine, pH 7.4, 150 mM NaCl
Recommended Dilutions ELISA, 1:1000-1:5000; WB, 1:1000-1:2000; IHC, 1:100-1:200
Species Reactivity Orai1-L1 antibody recognizes Orai1 from human and mouse. It may also recognize Orai2 and Orai3.

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