p53 protein acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are found in many types of cancers.
ELISA, WB, IHC, IP
Anti-TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.
Western blot analysis of recombinant TP53(R175H) and wildtype p53 proteins. Purified His-tagged TP53(R175H) protein (Cat. #10207) and wild type p53 protein were blotted with anti-TP53(R175H) rabbit antibody (Cat. #NEBA10072).
|Applications||ELISA, WB, IHC, IP|
|Purity||Purified from serum|
|Immunogen||A synthetic peptide from the internal region of p53 which includes the mutation of R175H, human origin|
|Storage Instructions||Store at -20°C. Avoid repeated freeze / thaw cycles.|
|Storage Buffer||Preservative: No; Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol|
|Recommended Dilutions||ELISA, 1:100-1:5000; WB, 1:100-1:2000; IHC, 1:50-1:100|
|Species Reactivity||Anti-TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.|