ATM (Phospho-Ser1981) Rabbit Polyclonal Antibody, 100 ug DBOA15118

ATM (Phospho-Ser1981) Rabbit Polyclonal Antibody, 100 ug Applications DBio DBOA15118 ATM (Phospho-Ser1981) Rabbit Polyclonal Antibody, 100 ug Applications DBio DBOA15118
ATM (Phospho-Ser1981) Rabbit Polyclonal Antibody, 100 ug Applications DBio DBOA15118
Supplier: DBio
Product Code: DBOA15118
Availability: In Stock

Background:

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.

Product datasheet:

Overview

Product Description   ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.
Tested Applications WB IHC
Image
Western blot analysis of extracts from Hela cells untreated or treated with UV using ATM (Phospho-Ser1981) Antibody #11122
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM (Phospho-Ser1981) Antibody #11122 (left) or the same antibody preincubated with blocking peptide (right).
Species Reactivities Human
Immunogen Peptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.

Properties

Storage Instructions Store at -20°C
Clonality Polyclonal Antibody

References:

  1. ATM Kinase Is Required for Telomere Elongation in Mouse and Human Cells. Lee SS, et al. Cell Rep, 2015 Nov 24. PMID 26586427 Free PMC Article
  2. Ataxia-telangiectasia mutated (ATM) participates in the regulation of ionizing radiation-induced cell death via MAPK14 in lung cancer H1299 cells. Liang N, et al. Cell Prolif, 2015 Oct. PMID 26269117
  3. [The role of ATM kinase in neurodegeneration]. Chwastek J, et al. Postepy Biochem, 2014. PMID 26263761
  4. Loss-of-function variants in ATM confer risk of gastric cancer. Helgason H, et al. Nat Genet, 2015 Aug. PMID 26098866
  5. Associations of ATM Polymorphisms With Survival in Advanced Esophageal Squamous Cell Carcinoma Patients Receiving Radiation Therapy. Du Z, et al. Int J Radiat Oncol Biol Phys, 2015 Sep 1. PMID 26094126
  6. The complete sequence of the coding region of the ATM gene reveals similarity to cell cycle regulators in different species.
    Savitsky K., Sfez S., Tagle D.A., Ziv Y., Sartiel A., Collins F.S., Shiloh Y., Rotman G.
    Hum. Mol. Genet. 4:2025-2032(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  7. The ATM gene and susceptibility to breast cancer: analysis of 38 breast tumors reveals no evidence for mutation.
    Vorechovsky I., Rasio D., Luo L., Monaco C., Hammarstroem L., Webster A.D.B., Zaloudik J., Barbanti-Brodano G., James M.R., Russo G., Croce C.M., Negrini M.
    Cancer Res. 56:2726-2732(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS CYS-49; ARG-1054; PHE-1420; ILE-2079 AND ALA-2287.
  8. Ataxia-telangiectasia locus: sequence analysis of 184 kb of human genomic DNA containing the entire ATM gene.
    Platzer M., Rotman G., Bauer D., Uziel T., Savitsky K., Bar-Shira A., Gilad S., Shiloh Y., Rosenthal A.
    Genome Res. 7:592-605(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  9. Human chromosome 11 DNA sequence and analysis including novel gene identification.
    Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.
    , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
    Nature 440:497-500(2006) [PubMed] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-1983.
  10. Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R.
    , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
General
NCBI Gene ID 472
Weight 350
Applications WB IHC
Component Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1.0mg/mL
Size 100 ug
Source Species Rabbit
Protein
Synonym AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Antibody
Specificity The antibody detects endogenous level of ATM only when phosphorylated at serine 1981.
Immunogen Peptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.
Clonality Polyclonal
Storage Instructions Store at -20°C
Recommended Dilutions WB 1:500~1:1000 IHC 1:50~1:100
Species Reactivity Human

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