A NEW Transfection Reagent developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. DNA-In™ Neuro is formulated specifically for maximum DNA delivery into neurons, providing high transfection efficiency and expression levels with very low toxicity.
Available in 4 sizes:
|Product Part No.||Size|
|MTIC3005X-73802||5 x 1.0 ml|
|MTIC3005X-73803||10 x 1.0 ml|
Mature cultures of neurons and other neural cells are extremely valuable for in vitro neurotoxicity studies and screening for agents that can slow, stop, or even reverse the course of neurodegenerative diseases.
The transfection of nucleic acids into neurons is essential for studying many aspects of neurobiology. However, neurons are among the most difficult cell types to transfect. They are very sensitive to culture conditions, presenting a particular challenge with regards to efficiencies. In addition to yielding low efficiencies, currently available cationic lipid reagents are often toxic to the cells, compromising post-transfection experimental results. While some viral mediated gene delivery systems have been shown to produce high efficiencies, they are very labor intensive and inconvenient for most researchers, along with the inherent danger and risk of provoking an immune response in the cell and/or interfering with the host genome.
ABOUT DNA-In™ Neuro
Developed by the co-inventors of the early Lipofectamine products, DNA-In™ Neuro was formulated from a novel chemistry for maximum transfection efficiency in neurons. In side-by-side assays with Lipofectamine 2000 significantly higher transfection efficiencies are consistently observed when DNA-In™ Neuro is used to transfect primary cortical, hippocampal or forebrain neurons. Moreover, with low cytotoxic effect DNA-In™ Neuro supports neuronal survival and neurite extension post-transfection.
DNA-In™ Neuro is a new transfection reagent that consistently produces high transfection efficiencies in neurons, typically achieving a 2-fold or better improvement in efficiency over the competing reagents currently available, including Lipofectamine® 2000 and NeuroFECT™. Moreover, DNA-In™ Neuro enables neurons to be efficiently transfected with minimal toxicity to support healthy post-transfected neurons, critical for performing assays on uncompromised transfected cells.
Figures 1 & 2. DNA-In Neuro Outperforms Leading Competitor Reagent– DNA-In™ Neuro was used to transfect plasmid DNA encoding GFP into 6-day cultured E18 Primary Rat Cortical Neurons. Transfections were performed in 24-well plates using 1.0-3.0µl of DNA-In™ Neuro Reagent. The above data show DNA-In™ Neuro significantly outperforms Lipofectamine® 2000 with near 3-fold improvement in transfection efficiency after 24 hours (Fig.1B). Duplicate wells were assayed 24-hrs and 48-hrs after transfection.
Figure 2. High GFP Expression in Primary Neurons – Primary rat cortical neurons were transfected with DNA-In™ Neuro Transfection Reagent and incubated overnight in complete culture media. The above images were taken 48-hours post-transfection and show uniform, high GFP expression in healthy cells.
DNA-In™ Neuro Transfection Reagent "Quick-Start Protocol"
DNA-In™ is a Molecular Transfer, Inc trademark
Lipofectamine®, Opti-MEM® are registered trademarks of Thermo Fisher Scientific Inc.
NeuroFECT™ is a Genlantis trademark
|Applications||DNA plasmid transfection, FOR RESEARCH USE ONLY - Not intended for human or animal diagnostic or therapeutic uses|
|Cell Types||Formulated specifcally for neurons|
|Size||Available in 4 sizes: 0.1 ml, 1.0 ml, 5x1.0 ml, 10x1.0 ml|
|Storage Instructions||Store at 4°C, Do Not Freeze|