DNA-In™ Transfection Reagent, 1.0ml (73751) MTIC30001

DNA-In™ Transfection Reagent, 1.0ml (73751) Cancer Research Molecular Transfer, Inc. MTIC30001
DNA-In™ Transfection Reagent, 1.0ml (73751) Cancer Research Molecular Transfer, Inc. MTIC30001
Supplier: Molecular Transfer, Inc.
Product Code: MTIC30001
Availability: In Stock

A New Universal Transfection Reagent, from Expert Developer Molecular Transfer Inc.

DNA-In™ is the newest generation transfection product developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000.  DNA-In™ is a novel cationic transfection reagent which balances high transfection and expression levels with low toxicity.  DNA-In™ works on a very broad range of adherent cells including most passaged cell lines and many primary cells.

Features

  • Fast, simple, easy-to-follow protocol
  • One-step process: simply add DNA-In™ Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells
  • Minimal toxicity - no need to replace medium after transfection


Product Documents


Superior Tranfection Efficiency

During its development and validation process, DNA-In™ Reagent underwent extensive testing in many primary and cultured cells.  In a head-to-head comparison study, DNA-In consistently outperformed the Lipofectamine 2000 transfection reagent.


 DNA-In outperforms Lipofectamine 2000 in GFP transfection

 DNA-In outperforms Lipofectamine 2000 in GFP transfection

 DNA-In outperforms Lipofectamine 2000 in GFP transfection

Cells were transfected with a plasmid, containing EmeraldGFP behind an EF1α promoter, with either DNA-In™ or Lipofectamine®2000, at several different lipid:DNA ratios and examined ~44 hours post-transfection. Representative images of the best conditions for each transfection reagent are shown.


Low Cell Toxicity 

DNA-In has a significantly lower cell toxicity compared to traditional transfection reagents such as Lipofectamine and Lipofectamine 2000.  In the many cell lines tested, DNA-In shows a broad range of effective dosage while maintaining minimal cell toxicity.  This low cell toxicity allows a larger dosage and time window for optimized transfection results.

The following charts show side-by-side comparisons of DNA-In and Lipofectamine 2000 (LF2K) in commonly used cell lines and primary cells:

DNA-In and Lipofectamine 2000 comparison in 293 cells

In 293 cells, DNA-In maintains high cell viability throughout the tested dosage range, while LF2K has a significant cell toxicity issue when used in dosages over 3 ul.


DNA-In and Lipofectamine 2000 comparison in A549 cells

In A549 cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.


DNA-In and Lipofectamine 2000 comparison in human fibroblasts

In human fibroblast cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.


DNA-In and Lipofectamine 2000 comparison in Hela cells
In Hela cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.
DNA-In and Lipofectamine 2000 in HepG2 cells
In HepG2 cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.
DNA-In and Lipofectamine 2000 comparison in MCF7 cells
In MCF7 cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K transfection efficiency is lower and is toxic when used in dosages higher than 2 ul.
 
Lipofectamine®, Opti-MEM® are registered trademarks of Thermo Fisher Scientific Inc.
 
General
Cell Types Hard-to-Transfect Cells, Primary Cells, Stem Cells, Commonly used cell lines
Size 1.0 ml

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