SH-SY5Y is a human derived neuroblastoma cell line commonly used in scientific research. It's a subclone of the original SK-N-SH cell line that was isolated from a bone marrow biopsy a female with neuroblastoma disease. SH-SY5Y cells are routinely used in studies related to neurotoxicity, oxidative stress and neurodegenerative diseases. Shown to express immature neuronal markers these cells are an established in vitro cell model of dopaminergic (DA) neurons for Parkinson's disease (PD) research.
DNA-In® SY5Y Transfection Reagent was optimized on the SH-SY5Y neuroblastoma cell line to give maximum transfection efficiency with exceptionally low cytotoxicity.
Optimized for high transfection efficiency in SH-SY5Y neuroblastoma cells.
One-step process: simply add DNA-In® SY5Y Transfection Reagent to diluted DNA, incubate 10 minutes, and to pre-plated cells
Maximum DNA transfection efficiency with minimal toxicity - no need to replace medium after transfection
Fast, simple, easy-to-follow protocol
Data below show side-by-side comparisons ofSH-SY5Y cells transfected with DNA-In® SY5Y Transfection Reagent vs competitor reagents:
Figure 1. SH-SY5Y neuroblastoma cells were plated in 24-well plates to give 60 -70% confluency the day of transfection. Cells were transfected with a plasmid, containing 500ng Emerald GFP behind an EF1α promoter, and various amounts of either DNA-In™ SY5Y, Lipofectamine®2000 (LF2K), or Lipofectamine®3000 ( LF3K). Above data shows significanly higher transfection efficiency in cells transfected with DNA-In SY5Y vs competitors. Images were captured at 10x magnification 24-hours after transfection.
SH-SY5Y neuroblastoma cells were plated in 24-well plates to give 60 -70% confluency the day of transfection. Cells were transfected with a plasmid, containing 500ng Emerald GFP behind an EF1α promoter, and various amounts of either DNA-In™ SY5Y, Lipofectamine®2000 (LF2K), or Lipofectamine®3000 ( LF3K). Relative fluorescent intensities were measured 24 hours post transfection using Tecan GENios at ex. 485nm and em. 535nm. Backgrounds were subtracted and the remaining values were plotted.
DNA-In® Is a registered trademark of Molecular Transfer, Inc.
Lipofectamine®, Opti-MEM® are registered trademarks of Thermo Fisher Scientific Inc.
|Cell Types||Formulated for SY5Y neuroblastoma cells|
|Size||0.1 ml, 1ml, 5x1 ml, 10,1 ml|