EPB41 (Phospho-Tyr660/418) Rabbit Polyclonal Antibody, 100 ug DBOA15495

EPB41 (Phospho-Tyr660/418) Rabbit Polyclonal Antibody, 100 ug Applications DBio DBOA15495
EPB41 (Phospho-Tyr660/418) Rabbit Polyclonal Antibody, 100 ug Applications DBio DBOA15495
Supplier: DBio
Product Code: DBOA15495
Availability: In Stock

Background:

The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes.

Product datasheet:

Overview

Product Description   Elliptocytosis is a hematologic disorder characterized by elliptically shaped erythrocytes and a variable degree of hemolytic anemia. Inherited as an autosomal dominant, elliptocytosis results from mutation in any one of several genes encoding proteins of the red cell membrane skeleton. The form discussed here is the one found in the 1950s to be linked to Rh blood group and more recently shown to be caused by a defect in protein 4.1. 'Rh-unlinked' forms of elliptocytosis are caused by mutation in the alpha-spectrin gene, the beta-spectrin gene, or the band 3 gene.
Tested Applications WB
Image
Western blot analysis of extracts from HepG2 cells treated with PMA using EPB41 (Phospho-Tyr660/418) Antibody #11799.The lane on the right is treated with the antigen-specific peptide.
Species Reactivities Human Mouse
Immunogen Peptide sequence around phosphorylation site of tyrosine 660/418 (N-I-Y(p)-I-R)derived from Human EPB41.

Properties

Storage Instructions Store at -20°C
Clonality Polyclonal Antibody

References:

  1. Comprehensive characterization of protein 4.1 expression in epithelium of large intestine. Zhang J, et al. Histochem Cell Biol, 2014 Nov. PMID 24912669
  2. Structural stabilization of protein 4.1R FERM domain upon binding to apo-calmodulin: novel insights into the biological significance of the calcium-independent binding of calmodulin to protein 4.1R. Nunomura W, et al. Biochem J, 2011 Dec 15. PMID 21848512
  3. Nonsense-mediated mRNA decay (NMD) blockage promotes nonsense mRNA stabilization in protein 4.1R deficient cells carrying the 4.1R Coimbra variant of hereditary elliptocytosis. Morinière M, et al. Blood Cells Mol Dis, 2010 Dec 15. PMID 20863723
  4. Identification of SNP markers on 1p36 and association analysis of EPB41 with mandibular prognathism in a Chinese population. Xue F, et al. Arch Oral Biol, 2010 Nov. PMID 20797695
  5. [Expression and significance of membrane skeleton protein 4.1 family in non-small cell lung cancer]. Zheng XY, et al. Ai Zheng, 2009 Jul. PMID 19624891
  6. Molecular cloning of protein 4.1, a major structural element of the human erythrocyte membrane skeleton.
    Conboy J.G., Kan Y.W., Shohet S.B., Mohandas N.
    Proc. Natl. Acad. Sci. U.S.A. 83:9512-9516(1986) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), PROTEIN SEQUENCE OF 378-393. Tissue: Reticulocyte.
  7. Expression of specific isoforms of protein 4.1 in erythroid and non-erythroid tissues.
    Tang T.K., Leto T.L., Marchesi V.T., Benz E.J. Jr.
    Adv. Exp. Med. Biol. 241:81-95(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5 AND 6).
  8. Selective expression of an erythroid-specific isoform of protein 4.1.
    Tang T.K., Leto T.L., Correas I., Alonso M.A., Marchesi V.T., Benz E.J. Jr.
    Proc. Natl. Acad. Sci. U.S.A. 85:3713-3717(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5 AND 6).
  9. Tissue- and development-specific alternative RNA splicing regulates expression of multiple isoforms of erythroid membrane protein 4.1.
    Conboy J.G., Chan J.Y.C., Chasis J.A., Kan Y.W., Mohandas N.
    J. Biol. Chem. 266:8273-8280(1991) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  10. Sequence of protein 4.1 from a human neuroblastoma cell line: LAN5.
    Huang S.C., Wang C., Lichtenauer U., Vortmeyer A., Zhuang Z.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
General
NCBI Gene ID 2035
Weight 60
Applications WB
Component Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1.0mg/mL
Size 100 ug
Source Species Rabbit
Protein
Synonym HE; EL1; 4.1R
Antibody
Specificity The antibody detects endogenous levels of EPB41 only when phosphorylated at tyrosine 660/418.
Immunogen Peptide sequence around phosphorylation site of tyrosine 660/418 (N-I-Y(p)-I-R)derived from Human EPB41.
Clonality Polyclonal
Storage Instructions Store at -20°C
Recommended Dilutions WB 1:500~1:1000
Species Reactivity Human Mouse

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