Melting Temperature (Tm) Calculation (DNA nearest-neighbor)

Primer Sequence (6-50 bases):

Primer concentration(unit:nM):

Salt concentration(unit:mM):

Mg2+ concentration(unit:mM):



LENGTH: 0
C+G% : 0
Molecular Weight: 0
Melting Temperature: 0
Enthalpy: 0
Entropy: 0

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