The Luna-FL™ is a dual fluorescence automated cell counter. It is a quantum leap for automated cell counting and cell viability analysis. The Luna-FL™ automated cell counter gives you the sensitive and accurate live/dead cell counting results without limitation of sample types.
The precision two-color fluorescence microscope optics, high resolution CCD imager and the new software algorithm were carefully integrated to achieve excellent cell counting accuracy. Within a 5x104 ~ 1x107 cells/ml concentration and 1 ~ 90 ㎛ cell diameter range, Luna™ software accurately detects live/dead cells, and discriminates cell debris. Individual cells in cell clusters are also successfully counted by Luna’s new de-clustering algorithm.
It is difficult to count primary cells such as PBMC, splenocytes, neutrophiles, and stem cells with the conventional cell counters such as Coulter counter or image-based automated cell counters utilizing only bright field optics. This is partly due the fact that primary cells are often contaminated with the undesirable debris which can be mis-recognized as cells with the conventional cell counters. Luna-FL™ is integrated with the dual fluorescene microscope optics to overcome this problem. Live/dead cells are stained with the green/red fluorescence dye (Acridine Orange / Propidium Iodide (AO/PI) cell viability kit, LGBD10012), and the fluorescence labled cell images are analyzed with the accurate image analysis software. To see a comparison between the Luna-FL and the Countess IITM in assessing primary cell viability, click here.
GFP Transfection Assay and Yeast Viability Assay
Luna-FL can be used to to measure GFP expression. Luna-FL calculates concentrations of total and GFP positive cells. It also provides average cell sizes and the GFP fluorescence intensity can be visualized/quantified on a histogram. For more information about using the Luna-FL to measure GFP expression click here to read our article or click here to download the pdf.
In addition to the AO/PI viability kit, A FDA/PI kit (LGBD10013) is also developed to measure yeast cell viability. The kit contains Fluorescein Diacetate (FDA), a plasma membrane permeable precursor of fluorescein. Once FDA enters live cells, it is converted into fluorescein by cellular esterase and is trapped in the live cells. For more information automated yeast cell counting, please read the article or download the pdf.
Minimal Counting Cost
Automated cell counters utilize disposable counting slides to eliminate washing steps of manual cell counting with glass hemocytometer.
Logos Biosystems has developed innovative T-Bond™ technology (patent-pending) to manufacture the precision cell counting slides more efficiently. The unit price of slide is almost half of the other brands, saving significant consumable costs for heavy users.
Interactive and Intuitive Software Interface
|A 2.5 min video showing features and operation of Luna-FL||A cool video of counting sperm cells while monitoring cell viability in real time!|
|Cell Concentration Range||5x10^4 - 1x10^7 cells/ml|
|Cell Counting Time||30 sec.|
|Cell Size Range||Detectable Range: 1-90 µm; Optimal Range: 5-60 µm|
|Emission Wavelength||525 +/- 25 nm, 600 nm (LP)|
|Excitation Light Source||LED|
|Excitation Wavelength||470 +/- 20 nm|
|Imaging Sensor||5 MP|
|LCD Display||7 inch (800 x 480 pixels)|
|Sample Volume||10 µl|
|Dimensions||22 x 21 x 9 cm (8.6 x 8.3 x 3.5 inch)|