The LUNA-II™ Automated Cell Counter is a small, fast, and affordable image-based cell counting device that automatically counts a variety of cell types.
The LUNA-II™ Automated Cell Counter is the latest cell counter model from Logos Biosystems and comes with an integrated package of:
The LUNA-II™ measures the number, as well as determining cell viability (live, dead, total cells) using sophisticated optical components and advanced image analysis algorithms. Due to several innovations introduced by Logos Biosystems, the LUNA-II™ provides a state of the art cell counting device and eliminates the tedium and subjectivity of manual cell counting.
Options for staining methods: The LUNA-II™ automated cell counter can count samples with or without trypan blue dye or Erythrosin B stain.
Cell size histogram and cell size based gating: After counting, cells may be gated based on their size. The specific size of cell populations may be easily visualized in a histogram.
Cell cluster map: Counted cells are identified using the cell cluster map that displays the percentage of single cells, doublets, or triplets. Cell cluster maps may be used to monitor changes in culture conditions or cell isolation/preparation protocols.
Review and Re-analysis option: The LUNA-II™ automated cell counter provides a powerful review and re-analysis option. To open and review the saved image, a separate PC is not necessary. Users can open the cell images directly on the LUNA-II™ automated cell counter, and can re-count cells using the different parameters.
Various file save options: The LUNA-II™ has an on-board memory to store up to 1,000 counts. After counting, the results are automatically saved in the memory. Previous counting results can be exported via the USB port as a .CSV file for further analysis. The results and image data may be saved as three different file formats such as a TIF, an annotated TIF, or a PDF report file. The PDF report file contains all the data generated during/after the cell counting, i.e., the protocol used, cell counting results, date, raw/analyzed cell image(s), and histograms are included in the PDF report file.
The LUNA™-II cell counting algorithm is recognized as having best-in-class cell counting accuracy. The LUNA™ software has exceptional accuracy in cell de-clustering and accurately counts 'clumpy' cells. The LUNA-II™ automated cell counter has inherited this well-known performance aspect of the LUNA™ software.
Cell Counting Data Provided by LUNA-II™:
The LUNA-II™ Automated Cell Counter is simple to use: First, mix 10 ul of the cell sample with 10 ul of trypan blue or Erythrosin B stain. Second, load the cell suspension into the Luna™ Cell Counting Slide. Third, insert the slide into the slide port of the instrument and chose [Autofocused Counting] or adjust the focus by clicking arrows to get an appropriate cell image. Last, press the [Count] button and the results of cell count and viability will display on the screen. The counting image may be downloaded onto a USB drive in TIF format for review and/or record keeping.
The Luna™ Cell Counting Slides are disposable and specifically designed for the LUNA-II™ Automated Cell Counter. Each counting slide has 2 chambers, labeled as A and B, respectively, so that one slide can be used for the same sample reading in duplicate or for 2 different samples if preferred.
Alternatively, LOGOS has developed a Reusable Slide that will allow you to cut down on waste.
|Cell Concentration Range||5x10^4 - 1x10^7 cells/ml (optimal range)|
|Cell Counting Time||Approx. 10 sec. (without autofocusing) or 15 sec. (with autofocusing) at 1 x 10^6 cell/ml concentration (processing time may vary by cell type and concentration)|
|Cell Size Range||Detectable Range: 3-60 µm; Optimal Range: 8-30 µm|
|Imaging Sensor||5 MP|
|LCD Display||5 inch (800 x 480 pixels)|
|Sample Volume||10 µl|
|Dimensions||16 x 18 x 28 cm (6.3 x 7.0 x 11.0 inch)|
|Weight||1.6 kg (3.5 lb) without the external power adaptor|