Molecular Biology Service

Site-directed Mutagenesis Service

Site-directed mutagenesis is essential to molecular biology in studies of gene regulatory elements, DNA-protein interaction, protein structure and its function, enzyme active sites and novel proteins. Our site-directed mutagenesis services include point mutations, deletions, and insertions. Our high quality work, quick delivery, low service prices and FREE shipment for local institutes, universities and companies are unbeatable.

Advantages

Mutation on Large Plasmid: Any point mutations, insertions and deletions can be made on up to 18 kb plasmid with our most advanced mutagenesis technology.
Highest Accuracy: Our high-fidelity DNA polymerase ensures accuracy of mutants and guarantees NO unwanted mutations.
Multiple mutation sites can be introduced in one mutagenesis reaction.
NO charges if we cannot deliver your mutant constructs.
Most Competitive price: Low service prices are unbeatable. NO HIDDEN FEES.
Sample Submission Requirements

Please provide following:

The complete sequence of the template (the target)
Mutation specifications
Maps and antibiotic resistance of the template and destination vectors
~5 μg of wildtype plasmid DNA
Delivery

Our delivery package includes:

2 - 4 ug of high pure mutant construct;
Project report with sequence alignments and original sequencing data (electronic).
Our typical turnaround is about 7 business days.
We offer FREE door-to-door delivery as well as sample collection for the customers in NIH Bethesda and Twinbrook campuses and other local Universities, Institutes and biotech companies.
Quotations and ordering

Random Mutagenesis

Random mutagenesis is a powerful tool to determine protein structure-function relationships and to improve or alter their characteristics by modifying proteins. Error-prone PCR is a random mutagenesis technique for generating amino acid substitutions in proteins by introducing mutations into a gene during PCR. Mutations are deliberately introduced by error- prone DNA polymerases. After the mutated PCR products are cloned into an expression vector, the resulting mutant library can be screened for changes in protein activity. Due to the lack of structural information, or when such mutations are difficult to predict from protein structure, the Scientists can use random mutagenesis to identify beneficial mutations. We offer to create a random mutagenesis library of any desired complexity to any gene of your interest.

Service Description:

Cloning of the randomized DNA into expression vectors.
Cloning into any other vectors.
Confirmation of desired mutation by DNA sequencing.
Selection of mutation frequencies depending on the application of the study.
Titration of your randomly mutated plasmid library.
Upon special request, we can provide library screening and protein activity assay, etc. with additional service fees.

Site-Directed Random Mutagenesis

Site-Directed Random Mutagenesis is also called Site-Saturation Mutagenesis or Targeted Random Mutagenesis or Domain Mutagenesis. Since conventional random mutagenesis generates large numbers of non-functional mutants rather than functional genes, these approaches inevitably require extensive screening over a long period of time, with a high cost outlay. By targeting specific residues or protein domains, most of the codons remain as wild-type, which can make the mutants maintain natural structures of protein backbones despite hyper diversity of the functional region. Therefore, the mutant library can contain a high portion of functional mutants that are most likely to yield positive results.

Service Description:

Cloning of the site-directed randomized DNA into expression vectors.
Cloning into any other vectors.
Confirmation of site-directed random mutations by DNA sequencing.
Selection of site-directed mutation frequencies depending on the application of the study.
Titration of your site-directed randomly mutated plasmid library.
Upon special request, we can provide library screening and protein activity assay, etc. with additional service fees.

Molecular Cloning Services

Cloning is not always straightforward to perform and can be a very time-consuming process. Our network of service labs has performed many cloning services that save companies and research laboratories time and money. Our molecular cloning services include PCR cloning, subcloning, custom vector constructions (e.g. knockout and knockin vector construction) and cloning genes (full-length cDNA) from total RNA or cDNA libraries. Our experienced scientists provide complete cloning services from project design to construction of clones, freeing you to spend more time on your high level research work. We stand behind everything we do with our 100% satisfaction guarantee.

How we are different from the competitors:

Large fragment subcloning: With our advanced techniques and cloning strategies, we have NO problems cloning large genes into big vectors (up to 18.0 kb).
Vector selection: Cloning can be done into any vector of your choice.
Minimum Turnaround Time: We can deliver customers' projects in around seven business days.
Competitive prices
Delivery

Our delivery package includes:

2 - 4 ug of high pure construct (plasmid DNA)
Project report with sequence alignments and original sequencing data (delivered as an electronic file).
*We offer FREE door-to-door delivery as well as sample collection for customers at NIH Bethesda and Twinbrook campuses, as well as other local Universities, Institutes and biotech companies. Please contact us for details.

Sample Submission Requirements

Please provide following:

The complete sequence of the template (the target gene)
Construct specifications
Maps and specification of antibiotic resistance of the template and destination vectors
~5 μg of plasmid DNA

Plasmid DNA Preparation Service

Our plasmid DNA preparation services have a flexible scale, from Miniprep (10 μg), MidiPrep (100 μg), Maxiprep (1-2 mg) up to a gram level. Our high quality plasmids will help you achieve highly efficient cell transfection, DNA vaccination and preclinical studies. 

Quality Grades
Research Grade:  For typical molecular biology research
Endo-toxin Free Grade:  Recommended for effective cell transfection, gene vaccine and protein manufacturing and animal studies 
GMP Plasmid DNA Grade: Our network of service labs has designed a tailored fermentation and purification process for the manufacture of your GMP plasmid DNA molecule. Our novel protocol yields up to a gram level of clinical grade plasmid DNA that can be used for DNA vaccines, gene therapy, or ex-vivo applications.

Advantages

Minimum Turnaround Time as fast as a few business days
Competitive Prices: Low service prices starting from $100 per MidiPrep (from 100 ml LB medium) and $250 per maxiprep (from 1 Liter LB medium)
Quality Control by sequence confirmation and restriction enzyme digestion (upon request; additional charges may apply)
Delivery

Our delivery package includes:

High-purity plasmid DNA product
Project report with quality control report (upon request)
*We offer FREE door-to-door delivery as well as sample collection for the customers located at the NIH Bethesda and Twinbrook campuses, and other local Universities, Institutes and biotech companies.

Sample Submission Requirements

Please provide following:

Plasmid copy number
Specification of antibiotic resistance of the plasmid
~1 μg of wildtype plasmid DNA

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