mRNA-In™ Transfection Reagent was developed by members of the scientific team that invented Lipofectamine® and Lipofectamine® 2000 and specifically formulated forin vitro mRNA delivery. Maximum RNA delivery is achieved with mRNA-In™ using less amount mRNA than other reagents, reducing experimental costs and significantly lowering toxicity. Across a wide range of cell types, mRNA-In™ has shown to produce exceptionally high transfection efficiency while maintaining optimal cell health and viability.
mRNA-In™ Transfection Reagent is ideal for mRNA delivery for various applications, such as protein expression and iPSC-reprogramming. Data below also shows mRNA-In™ significantly outperforms competitor reagents, typically achieving 90% or greater transfection efficiency across a wide range of cell types.
mRNA-In™ Delivers Maximum Transfection Efficiency using Low Amounts of mRNA Across a Range of Cell Types
Data below show commonly used cell types transfected with mRNA-In™ Transfection Reagent using various amounts of mRNA. Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with various amounts of mRNA-In™ in a total of 50µl of OptiMEM. For each experiment mRNA /mRNA-In™ complexes were added to cells, mixed, and incubated at 37ºC in 5% CO2. Cells were observed 24 hours following transfection.
|SH-SY5Y Neuroblastoma Cells were transfected with100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent||Human Adult Fibroblasts were transfected with 200ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent||HUVEC cells were transfected with100ng GFP-mRNA/1µL mRNA-In™ Reagent Transfection Reagent|
|iPSC-Derived Neural Stem Cells were transfected with 50ng GFP-mRNA /0.25µL mRNA-In™ Transfection Reagent||NCRM-1 iPSC were transfected with 100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent|
mRNA-In™ Delivers Higher GFP Expression and Outperforms the Leading Competitor Transfection Reagent
Below, SH-Sy5Y (neuroblastoma cells), HDF (human dermal fibroblasts), HUVEC (human umbilical vein endothelial cells), HeLa and adiposed-derived Mesenchymal Stem Cells (MSCs) were transfected with mRNA-In™ or MessengerMAX™ (Life Technologies) using low amounts of mRNA (100ng). Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with the various amounts of mRNA-In™ (see chart legends). RNA/reagent complexes were added to the cells, mixed, and incubated at 37ºC in 5% CO2 overnight. Cells were analyzed by a fluorescence plate reader and microscopy 24 hours post-transfection. Error bars represent the standard deviation of triplicate wells. The data below shows mRNA-In™ provides significantly higher transfection efficiency than the competitor reagent.
|Cell Types||Formulated for most commonly used cell lines and primary cells.|
|Size||Various sizes: 0.1ml/1ml/5ml/10ml|