mRNA-In™ mRNA Transfection Reagent - Various Sizes MTIC3106X

mRNA-In™ mRNA Transfection Reagent - Various Sizes Cancer Research Molecular Transfer, Inc. MTIC3006X
Supplier: Molecular Transfer, Inc.
Product Code: MTIC3106X
Availability: In Stock

Available Options

* Size:





NEW! - A Universal Transfection Reagent for High Efficiency mRNA Delivery

mRNA-In™ Transfection Reagent was developed by members of the scientific team that invented Lipofectamine® and Lipofectamine® 2000 and specifically formulated forin vitro mRNA delivery. Maximum RNA delivery is achieved with mRNA-In™ using less amount mRNA than other reagents, reducing experimental costs and significantly lowering toxicity. Across a wide range of cell types, mRNA-In™ has shown to produce exceptionally high transfection efficiency while maintaining optimal cell health and viability. 

Features:

  • Requires low amounts of RNA - reduce cost and potential off-target toxicity effects with low amounts of RNA requirements.
  • High expression, low toxicity - achieve high mRNA efficiency while maintaining optimal cell health for uncompromised experiment results.
  • Robust, versatile performance - produce efficient transfection across a wide range of cell type, including stem cells and primary cells.

  mRNA-In™ Transfection Reagent for high efficiency RNA delivery Easy-to-follow "Quick" protocol 

 

 

 

Application:

mRNA-In™ Transfection Reagent is ideal for mRNA delivery for various applications, such as protein expression and iPSC-reprogramming. Data below also shows mRNA-In™ significantly outperforms competitor reagents, typically achieving 90% or greater transfection efficiency across a wide range of cell types.

mRNA-In™ Delivers Maximum Transfection Efficiency using Low Amounts of mRNA Across a Range of Cell Types
Data below show commonly used cell types transfected with mRNA-In™ Transfection Reagent using various amounts of mRNA. Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with various amounts of mRNA-In™ in a total of 50µl of OptiMEM.  For each experiment mRNA /mRNA-In™ complexes were added to cells, mixed, and incubated at 37ºC in 5% CO2.  Cells were observed 24 hours following transfection.

SH-Sy5Y Neuroblastoma  RNA transfection Human dermal fibroblast mRNA transfection HUVEC (human umbilical vein endothelial cord) RNA transfection
SH-SY5Y Neuroblastoma Cells were transfected with100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent Human Adult Fibroblasts were transfected with 200ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent  HUVEC cells were transfected with100ng GFP-mRNA/1µL   mRNA-In™ Reagent Transfection Reagent  
     
Neural stem cell (NSC) RNA transfection iPSC (induced pluripotent stem cell)  RNA transfection during reprogramming  
iPSC-Derived Neural Stem Cells were transfected with 50ng GFP-mRNA /0.25µL mRNA-In™ Transfection Reagent               NCRM-1 iPSC were transfected with 100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent 

 

mRNA-In™ Delivers Higher GFP Expression and Outperforms the Leading Competitor Transfection Reagent
Below, SH-Sy5Y (neuroblastoma cells), HDF (human dermal fibroblasts), HUVEC (human umbilical vein endothelial cells), HeLa and adiposed-derived Mesenchymal Stem Cells (MSCs) were transfected with mRNA-In™ or MessengerMAX™ (Life Technologies) using low amounts of mRNA (100ng). Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with the various amounts of mRNA-In™ (see chart legends). RNA/reagent complexes were added to the cells, mixed, and incubated at 37ºC in 5% CO2 overnight.  Cells were analyzed by a fluorescence plate reader and microscopy 24 hours post-transfection. Error bars represent the standard deviation of triplicate wells. The data below shows mRNA-In™ provides significantly higher transfection efficiency than the competitor reagent.

SH-SY5Y neuroblastoma cell transfected with 100 ng mRNA with mRNA-InmRNA-In™ transfected SH-Sy5Y neuroblastoma cells

mRNA-In™ transfected human fibroblasts

HUVEC-100ng-mRNA with mRNA-In mRNA-In™ transfected HUVEC

HeLa 100 ng mRNA transfection with mRNA-InmRNA-In™ transfected HeLa

Adipose MSC transfected with 100 ng mRNA with mRNA-InmRNA-In™ transfected mesenchymal stem cells (MSC)

General
Cell Types Formulated for most commonly used cell lines and primary cells.
Size Various sizes: 0.1ml/1ml/5ml/10ml

Write a review

Your Name:


Your Review: Note: HTML is not translated!

Rating: Bad           Good

Enter the code in the box below:



©BioAstrum Corporation. All Rights Reserved 2014.
Google+