mRNA-In™ Neuro, is a new, mRNA transfection reagent specifically designed and optimized for high efficiency mRNA delivery with exceptionally low cytotoxicity in neurons. Unlike other competitor reagents, mRNA-In Neuro requires very low amounts of RNA to achieve maximum delivery while maintaining optimal cell viability.
NEW mRNA-In™ Neuro Transfection Reagent is a next generation low-toxicity mRNA delivery system for achieving maximum transfection efficiency in hard to transfection neurons. It is well suited for various neurobiology applications, including neurotoxicity and neurite outgrowth development. Data here shows mRNA-In™ typically achieves 70% or greater transfection efficiency in primary neurons. With mRNA-In Neuro, mRNA expression levels using can be adjusted by changing the amount of mRNA transfection. Moreover, mRNA-In™ - Neuro requires very low amounts of mRNA (up to 5-fold less that competitor reagents) to achieve maximum transfection efficiency in neurons, significantly minimizing unintended off-target immune activation and toxicity effects.
Low Amounts of mRNA Achieve Maximum RNA Delivery in Neurons
|500 ng mRNA||250 ng mRNA||120 ng mRNA|
Figure 1. Superior mRNA Transfection efficiency in Primary Neurons – Primary rat cortical neurons cultured in 24-well plates were transfecte with mRNA-In™ Neuro Transfection Reagent and incubated overnight in complete culture media. Maximum transfection efficiency was maintained with reducing amounts of mRNA, from 500ng (high) to 125ng (low) amounts of mRNA. The above images were taken 48-hours post-transfection.. In contrast to DNA transfection, neurons transfected with mRNA-In™ Neuro showed uniform GFP expression from high (500ng) to very low (125ng) amounts of mRNA.
Maximum efficiency with low amounts of mRNA - Maximally effective with very low amounts of mRNA, significantly minimizing potential adverse off-target cytotoxic effects and supporting maximum cell health and viability.
mRNA-In™ Neuro Shows Superior Performance over Competitor
Figure 2. mRNA-In™ Neuro Outperforms Competitor Reagents - mRNA-In Neuro Transfection Reagent was compared to MessengerMax™ for transfecting neurons. Primary cortical neurons in culture for 6 days were transfected with 500ng and 250ng amounts of modified mRNA and 2µl mRNA-In™ Neuro or MessengerMax™ in 24-well plates. Cells were incubated overnight in complete culture media and observed 24hr and 48hrs (not shown) post-transfection. Above images show significantly higher transfection efficiency and GFP expression in healthy neurons post transfection.
Messenger RNA Delivery in Neurons
Messenger RNA (mRNA) delivery has become a widely used non-viral gene delivery alternative due to its many advantages, including no risk of integration into the host genome which can lead to insertional mutagenesis and activation of oncogenes, and as shown here the ability to achieve very high transfection efficiencies, nearing that of viral delivery methods. In contrast with DNA transfection, mRNA delivery does not enter the nucleus, rather only cytosolic delivery is required, resulting in more a rapid expression. mRNA-In™ Neuro, is specifically designed and optimized for high efficiency mRNA delivery with exceptionally low cytotoxicity in neurons. Unlike all other competitor reagent mRNA-In™ Neuro requires very low amounts of RNA to achieve maximum delivery while maintaining optimal cell viability.
|Cell Types||Formulated for neurons|
|Size||Various sizes: 0.1 ml/1ml/5ml/10ml|