There are many examples of how nanotopographic structures throughout tissues and organs within multi-cellular organisms influence cellular behavior.
With lithographic manufacturing technology, it now is possible to precisely fabricate biomimetic nanotopographic structures within a cell culture device that produce a more 'natural' cellular behavior within a cell population.
Numerous in vitro studies utilizing fabricated nanotopograhic features demonstrate how biomimetic nanostructures impact cellular behaviors (e.g. cell morphology and cell differentiation) and physiology. The following figure shows how cells exhibit different morphologies depending on whether or not the culture surface is flat or grooved.
Nanostructure with 800 nm wide, 600 nm ridges/grooves is created with nanofabrication.
Human iPSC derived cardiomyocytes (left panel) and Endothelial cells (EA.hy926 human ECs) (Right Panel) cultures grown on either a flat or ANFS surface.
|1||Cardiomyocytes||Kim et al., PNAS. 2010, 107(2), 565-570|
|2||Human iPSC derived cardiomyocytes||Macadangdang et al, Cellular and Molecular Bioengineering. 2015, 8(3), 320-332|
|3||Smooth muscle cells||Nemeth et al. Tissue Engineering: Part A. 20(21 and 22), 2817-2829|
|4||Endothelial cells (EA.hy926 human ECs)||Jeon et al. ACS Applied Materials and Interfaces. 2015, 4525-4531|
|5||NIH 3T3 fibroblast cells||Kim et al. Biomaterials, 2009, 30(29), 5433–5444|
|6||Dental pulp stem cells||Nemeth et al. Tissue Engineering: Part A. 20(21 and 22), 2817-2829|
|7||Human mesenchymal stem cells||J. Kim et al. Scientific Reports 3, Article number: 3552 (2013)|
|8||Pre-adipocytes (3T3-L1)||M.S. Kim et al Nanotechnology Journal, Volume 22, Issue Number 49|