NanoSurface ANFS 35mm Cell Culture Dish, ANFS nano surface, sterile, 10, 30 or 50 dishes/pack NSBF90010

NanoSurface ANFS 35mm Cell Culture Dish, ANFS nano surface, sterile, 10, 30 or 50 dishes/pack Lab Supplies NanoSurface Biomedical, Inc. NSBF90010 NanoSurface ANFS 35mm Cell Culture Dish, ANFS nano surface, sterile, 10, 30 or 50 dishes/pack Lab Supplies NanoSurface Biomedical, Inc. NSBF90010
NanoSurface ANFS 35mm Cell Culture Dish, ANFS nano surface, sterile, 10, 30 or 50 dishes/pack Lab Supplies NanoSurface Biomedical, Inc. NSBF90010
Supplier: NanoSurface Biomedical, Inc.
Product Code: NSBF90010
Availability: In Stock

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NanoSurface ANFS 35mm Cell Culture Dish, ANFS nano surface, sterile, 10, 30 or 50 dishes/pack

Features.

  • Nanopattern with 800nm 1:1 Ridge to Groove Spacing
  • Modulus range close to that of overall muscle tissue (6.69 MPa)
  • Bio-compatible PUA
  • Dish size: 35 mm
  • Hole size: 20Ø
  • Plate size: 6, 24 or 96 well plate
  • Gamma sterilized

Functional Test.

  • Sterility Passed
  • Cytotoxicity Passed
  • Non-Pyrogenic Passed
  • RNase/DNase Free
     


     

    Background


    There are many examples of how nanotopographic structures throughout tissues and organs within multi-cellular organisms influence cellular behavior.

    With lithographic manufacturing technology, it now is possible to precisely fabricate biomimetic nanotopographic structures within a cell culture device that produce a more 'natural' cellular behavior within a cell population.

    Numerous in vitro studies utilizing fabricated nanotopograhic features demonstrate how biomimetic nanostructures impact cellular behaviors (e.g. cell morphology and cell differentiation) and physiology. The following figure shows how cells exhibit different morphologies depending on whether or not the culture surface is flat or grooved.


     

    ANFS (Anisotropically NanoFabricated Substratum) Technology

     

    Nanostructure with 800 nm wide, 600 nm ridges/grooves is created with nanofabrication.


     

    ANFS Cell/Tissue Culture Dish and Plates

     


     

    Cell and Tissue Culture with ANFS Surface

     

    Human iPSC derived cardiomyocytes (left panel) and Endothelial cells (EA.hy926 human ECs) (Right Panel) cultures grown on either a flat or ANFS surface.


     

    Easy to Use ANFS Cell Culture Protocol

     


     

    Documents

     

    ANFS Technology Brochure

    ANFS Cell Culture Products Brochure

    ANFS dish and 6 Well Plate Product Sheet

    Published Cell Adhesion Data on ANFS Surface

    Nanosurface Cell Culture Protocol


     

    Publication References

     
      Cell Type Publication
    1 Cardiomyocytes Kim et al., PNAS. 2010, 107(2), 565-570
    2 Human iPSC derived cardiomyocytes Macadangdang et al, Cellular and Molecular Bioengineering. 2015, 8(3), 320-332
    3 Smooth muscle cells Nemeth et al. Tissue Engineering: Part A. 20(21 and 22), 2817-2829
    4 Endothelial cells (EA.hy926 human ECs) Jeon et al. ACS Applied Materials and Interfaces. 2015, 4525-4531
    5 NIH 3T3 fibroblast cells Kim et al. Biomaterials, 2009, 30(29), 5433–5444
    6 Dental pulp stem cells Nemeth et al. Tissue Engineering: Part A. 20(21 and 22), 2817-2829
    7 Human mesenchymal stem cells J. Kim et al. Scientific Reports 3, Article number: 3552 (2013)
    8 Pre-adipocytes (3T3-L1) M.S. Kim et al Nanotechnology Journal, Volume 22, Issue Number 49

     

     


 

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