GSK3β (Phospho-Ser9) Rabbit Polyclonal Antibody, 100 ug DBOA15001

Phospho-Histone H2A (T121) Polyclonal Antibody, 100µl, (12381) Phospho-Histone H2A (T121) Polyclonal Antibody, 100µl, (12381) Phospho-Histone H2A (T121) Polyclonal Antibody, 100µl, (12381) Phospho-Histone H2A (T121) Polyclonal Antibody, 100µl, (12381) Phospho-Histone H2A (T121) Polyclonal Antibody, 100µl, (12381) Phospho-Histone H2A (T121) Polyclonal Antibody, 100µl, (12381)
Phospho-Histone H2A (T121) Polyclonal Antibody, 100µl, (12381)
Supplier: DBio
Product Code: DBOA15001
Availability: In Stock

Background:

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity. Phosphorylates ZC3HAV1 which enhances its antiviral activity. Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation. Phosphorylates SFPQ at 'Thr-687' upon T-cell activation. Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Regulates the circadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2. Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation. Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation. Phosphorylates OGT at 'Ser-3' or 'Ser-4' which positively regulates its activity. Phosphorylates MYCN in neuroblastoma cells which may promote its degradation (PubMed:24391509).

Product datasheet:

Overview

Product Description   Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Tested Applications WB IHC IF
Image
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3β (Phospho-Ser9) Antibody #11002.
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #11002.
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002 (left) or the same antibody preincubated with blocking peptide #51002 (right).
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002.
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GSK3β (Phospho-Ser9) Antibody #11002.
Species Reactivities Human Mouse Rat
Immunogen Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β.

Properties

Storage Instructions Store at -20°C
Clonality Polyclonal Antibody

References:

  1. [Association between GSK3β polymorphisms and the smoking habits in young Japanese]. Nagahori K, et al. Nihon Shinkei Seishin Yakurigaku Zasshi, 2015 Jun. PMID 26281299
  2. Lenticular cytoprotection, part 2: link between glycogen synthase kinase-3β, epithelial to mesenchymal transition, and mitochondrial depolarization. Neelam S, et al. Mol Vis, 2014. PMID 25593505 Free PMC Article
  3. [Oxidative stress promotes hepatocyte apoptosis mediated by glycogen synthase kinase 3β]. Zhang X, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2015 Jan. PMID 25575054
  4. GSK3β is increased in adipose tissue and skeletal muscle from women with gestational diabetes where it regulates the inflammatory response. Lappas M. PLoS One, 2014. PMID 25541965 Free PMC Article
  5. Clinical significance of inactivated glycogen synthase kinase 3β in HPV-associated cervical cancer: Relationship with Wnt/β-catenin pathway activation. Rath G, et al. Am J Reprod Immunol, 2015 May. PMID 25532422
  6. Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.
    Stambolic V., Woodgett J.R.
    Biochem. J. 303:701-704(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), MUTAGENESIS OF SER-9.
  7. Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R.
    , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Placenta.
  9. Molecular cloning and characterization of the human glycogen synthase kinase-3beta promoter.
    Lau K.F., Miller C.C.J., Anderton B.H., Shaw P.C.
    Genomics 60:121-128(1999) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
  10. Radiation hybrid mapping of genes in the lithium-sensitive wnt signaling pathway.
    Rhoads A.R., Karkera J.D., Detera-Wadleigh S.D.
    Mol. Psychiatry 4:437-442(1999) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 185-202.
General
NCBI Gene ID 2932
Protein Name Histone H2A type 1-B/E
SWISS-Prot ID P0C0S8/P04908/P20671/Q96KK5/Q6FI13/Q7L7L0, P22752/Q8CGP6, P02262
Weight 46
Gene Symbol HIST1H2AB
Applications WB IHC IF
Component Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1.0mg/mL
Size 100 ug
Storage and shelf-life -20°C/1 year
Source Species Rabbit
Protein
Synonym H2A/c; H2AFC
Antibody
Antigen Modification Phosphorylation
Specificity The antibody detects endogenous level of GSK3β only when phosphorylated at serine 9.
Immunogen Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β.
Clonality Polyclonal
Storage Instructions Store at -20°C
Recommended Dilutions WB 1:500~1:1000 IHC 1:50~1:100
Purification Notes The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Species Reactivity Human Mouse Rat

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