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mRNA-In™ Transfection Reagent is a member of the newest generation transfection products developed by the lead members of the scientific team that invented and developed Lipofectamine® and Lipofectamine® 2000. mRNA-In™ produces exceptionally high transfection efficiency and expression levels with low toxicity across a broad range of cell types wtih even the lowest amount of RNA, further reducing costs and potential off-target toxicity.
|SH-SY5Y Neuroblastoma Cells were transfected with100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent||Human Adult Fibroblasts were transfected with 200ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent||HUVEC cells were transfected with100ng GFP-mRNA/1µL mRNA-In™ Reagent Transfection Reagent|
|iPSC-Derived Neural Stem Cells were transfected with 50ng GFP-mRNA /0.25µL mRNA-In™ Transfection Reagent||NCRM-1 iPSC were transfected with 100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent|
mRNA-In™ Delivers Maximum Transfection Efficiency using Low Amounts of mRNA Across a Range of Cell Types
Data below show commonly used cell types transfected with mRNA-In™ Transfection Reagent using various amounts of mRNA. Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with various amounts of mRNA-In™ in a total of 50µl of OptiMEM. mRNA /mRNA-In™ complexes were added to cells, mixed, and incubated at 37ºC and observed at 24 hours post-transfection.