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mRNA Delivery

NOW AVAILABLE! -  A New Universal mRNA Transfection Reagent 

mRNA-In™ Transfection Reagent is a member of the newest generation transfection products developed by the lead members of the scientific team that invented and developed Lipofectamine® and Lipofectamine® 2000. mRNA-In™ produces exceptionally high transfection efficiency and expression levels with low toxicity across a broad range of cell types wtih even the lowest amount of RNA, further reducing costs and potential off-target toxicity.

SH-Sy5Y Neuroblastoma  RNA transfection Human dermal fibroblast mRNA transfection HUVEC (human umbilical vein endothelial cord) RNA transfection
SH-SY5Y Neuroblastoma Cells were transfected with100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent Human Adult Fibroblasts were transfected with 200ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent  HUVEC cells were transfected with100ng GFP-mRNA/1µL   mRNA-In™ Reagent Transfection Reagent  
Neural stem cell (NSC) RNA transfection iPSC (induced pluripotent stem cell)  RNA transfection during reprogramming  
iPSC-Derived Neural Stem Cells were transfected with 50ng GFP-mRNA /0.25µL mRNA-In™ Transfection Reagent               NCRM-1 iPSC were transfected with 100ng GFP-mRNA/1µL mRNA-In™ Transfection Reagent 

mRNA-In™ Delivers Maximum Transfection Efficiency using Low Amounts of mRNA Across a Range of Cell Types

Data below show commonly used cell types transfected with mRNA-In™ Transfection Reagent using various amounts of mRNA. Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNA containing 5-methlycytosine and psuedouridine were complexed with various amounts of mRNA-In™  in a total of 50µl of OptiMEM.  mRNA /mRNA-In™ complexes were added to cells, mixed, and incubated at 37ºC and observed at 24 hours post-transfection.

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mRNA, Delivery, Transfection, Reagents
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